Filter
Add to wishlist

Enzyme- and Transporter-Based Drug-Drug Interactions: Progress and Future Challenges eBook

$14.00

  • Delivery: Can be download immediately after purchasing. For new customer, we need process for verification from 30 mins to 24 hours.
  • Version: PDF/EPUB. If you need another version, please Contact us
  • Quality: Full page, full content, high quality images, searchable text and you can print it.
  • Compatible Devices: Can be read on any devices (Kindle, NOOK, Android/IOS devices, Windows, MAC,..).
  • e-Book Features: Purchase and read your book immediately, access your eTextbook anytime and anywhere, unlimited download and share with friends.
  • Note: If you do not receive the download link within 15 minutes of your purchase, please Contact us. Thank you!
  • 5% off all orders over $25 with code:: 5OFF2024
  • 10% off all orders over $50 with code: 10OFF2024
SKU: 9781441908391 Categories: ,

An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatenation, and ATP hydrolysis. Several modified DNA substrates, used to dissect enzyme mechanisms by trapping intermediates, are also described. Methodologies to determine the action of topoisomerase-targeted drugs include biochemical assays of drug-induced enzyme-DNA complexes, methods for assaying drug uptake, and cell-based assays for determining the specificity and mechanisms of drug resistance. A companion volume, DNA Topoisomerase Protocols, I: DNA Topology and Enzymes, provides state-of-the-art experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function.