Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.
Drug Metabolism Enzymes eBook
$12.00
- Delivery: Can be download immediately after purchasing. For new customer, we need process for verification from 30 mins to 24 hours.
- Version: PDF/EPUB. If you need another version, please Contact us
- Quality: Full page, full content, high quality images, searchable text and you can print it.
- Compatible Devices: Can be read on any devices (Kindle, NOOK, Android/IOS devices, Windows, MAC,..).
- e-Book Features: Purchase and read your book immediately, access your eTextbook anytime and anywhere, unlimited download and share with friends.
- Note: If you do not receive the download link within 15 minutes of your purchase, please Contact us. Thank you!
- 5% off all orders over $25 with code:: 5OFF2024
- 10% off all orders over $50 with code: 10OFF2024